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Effect of three different irrigation solutions applied by passive ultrasonic irrigation

Restorative Dentistry & Endodontics 2015³â 40±Ç 2È£ p.143 ~ 148
Llena Carmen, Forner Leopoldo, Cambralla Raquel, Lozano Adrian,
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 ( Llena Carmen ) - Universitat de Valencia Department of Stomatology
 ( Forner Leopoldo ) - Universitat de Valencia Department of Stomatology
 ( Cambralla Raquel ) - Private practice
 ( Lozano Adrian ) - Universitat de Valencia Department of Stomatology

Abstract


Objectives: This study evaluated the maximum depth and percentage of irrigant penetration into dentinal tubules by passive ultrasonic irrigation (PUI).

Materials and Methods: Thirty extracted human teeth were instrumented and divided into three groups. According to final irrigation regimen, 5.25% sodium hypochlorite (Group A, NaOCl), 2% chlorhexidine (Group B, CHX) and saline solution (Group C, control group) were applied with Irrisafe 20 tips (Acteon) and PUI. Irrigant was mixed with 0.1% rhodamine B. Sections at 2 mm, 5 mm, and 8 mm from the apex were examined with confocal laser scanning microscopy (CLSM). The percentage and maximum depth of irrigant penetration were measured. Kruskal-Wallis test and Mann-Whitney test were performed for overall comparison between groups at each level and for pairwise comparison, respectively. Within a group, Wilcoxon test was performed among different levels. p values less than 0.05 were considered significant.

Results: In all groups, highest penetration depth and percentage of penetration were observed at the 8 mm level. At 2 mm level, Groups A and B had significantly greater depths and percentages in penetration than Group C (p < 0.05), but there were no significant differences between Groups A and B. At 5 mm level, penetration depths and percentage of penetration was not significantly different among the groups.

Conclusions: NaOCl and CHX applied by PUI showed similar depth and percentage of penetration at all evaluated levels.

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Chlorhexidine; Confocal laser scanning microscope; Dentinal penetration; Passive ultrasonic irrigation; Sodium hypochlorite

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